Physiological evaluation for the effect of cinnamon and turmeric dressing bandages on rheumatoid arthritis induced experimentally in rats

Abstract

Rheumatoid arthritis (RA) is a long-term ongoing condition where the immune system attacks the tissues lining a joint(s) of body especially in hands and feet which resulting in swelling, stiffness, as well as structural and functional alterations of affected joints and /or other organs or systems of the body. This study aims experimentally to evaluate the effects of cinnamon, turmeric, and both (cinnamon and turmeric) dressing bandages in amelioration the impact RA through measurement of some related serum markers. Thus, a total of 50 adult male rats were purchased, acclimated, and divided equally into five groups as following; PC (CFA-injected, not-treated), NC (neither-injected, nor-treated), CEG (CFA-injected, cinnamon-treated), TEG (CFA-injected, turmeric-treated), and CTEG (CFA-injected, cinnamon and turmeric-treated). Post 30 days, blood were samples directly from heart, and the obtained sera were tested using the quantitative ELISA to measurement the levels of amyloid A (AA), anti-cyclic citrullinated peptide antibody (Anti-CCP-Ab), cross linked C-telopeptide of type I collagen (CTX-I), interleukin-6 (IL-6), matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-10 (MMP-10), rheumatoid factor (RF), and tumor necrosis factor-alpha (TNF-α). GraphPad Prism Software was applied to indicate significant differences between the values of the five study groups at p<0.05. In comparison to values of PC, the findings of experimentally study groups (CEG, TEG, and CTEG) were revealed a significant reduction in values of all study markers (AA, Anti-CCP-Ab, CTX-I, IL-6, MMP-1, MMP-10, RF and TNF-α); however, the values of these markers were remained higher than detected in rats of NC group. Among the findings of experimentally study groups, the highest values of AA, Anti-CCP-Ab, CTX-I, IL-6, MMP-1, RF and TNF-α were detected in rats of CEG while the lowest were identified in CTEG when compared to values of TEG. Nonetheless, values of MMP-10 indicated no significant differences were found between the rats of experimentally groups. In conclusion, though dressing bandages containing turmeric are more activity than cinnamon, dressing bandages composed both cinnamon and turmeric were highly active in amelioration the negative impact of RA than those covered solely with cinnamon or turmeric. Additionally, the studied markers can be used definitively in diagnosing of RA and estimation the severity of infection especially at acute phase of disease. However, furthermore studies on dressing bandages of cinnamon and turmeric are greatly required due to the low number of available data and investigating their effects on other serum markers